Improvement of Glucose Homeostasis and Hepatoprotective Effects of Ganoderma applanatum Polysaccharides Extract in MACAPOS 2 Obese Rats

Author(s): Mfopa Adamou, Njimona Ibrahim, Clémence Mvongo, Mountchissi Célestin, Moustapha, Nkoubat Tchoundjwen Sandrine, Joseph Ngakou Mukam, Paul Aimé Noubissi, Eugène Sobngwi, René Kamgang, #Emmanuel Eroume A Egom, #Ngondi Judith Laure

The hyperglycaemia and hepatic steatosis are metabolic complications associated with obesity. This study was designed to investigate the glucose homeostasis improvement and hepatoprotective effects of Ganoderma applanatum polysaccharides (GAPs) extract in MACAPOS 2 obese rats. Obesity was induced in 6-8 week-old Wistar rats with a local high-fat diet for 16 weeks. The GAPs extract was orally administered to obese rats for two months at different doses (50, 100, and 150 mg/ kg body weight). At the end of the study, the effects of GAPs extract were evaluated on blood glucose through oral glucose tolerance, insulin tolerance, and fasting blood glucose tests. Then, the hepatoprotective properties through lipid peroxidation (malondialdehyde and hydroperoxide), antioxidant activities (SOD, CAT, GPx), cytolysis function (ASAT, ALAT), and liver histological structure. The results showed that GAPs extract improved (reduces) glucose levels after the oral glucose tolerance test and significantly reduced blood glucose. Moreover, the administration of GAPs extract improved the liver architecture and significantly reduced hepatic malondialdehyde and hydroperoxide. ALAT and ASAT activities were significantly reduced whereas hepatic superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) activities were significantly increased in GAPs-treated groups. The GAPs extract administered at different doses showed beneficial effects on glucose homeostasis, and hepatoprotective potential in MACAPOS 2 obese rats.